diff --git a/chrome/browser/about_flags.cc b/chrome/browser/about_flags.cc
index decfafa7da07d..d13c1835407ca 100644
--- a/chrome/browser/about_flags.cc
+++ b/chrome/browser/about_flags.cc
@@ -3635,9 +3635,6 @@ const FeatureEntry kFeatureEntries[] = {
     {"mac-syscall-sandbox", flag_descriptions::kMacSyscallSandboxName,
      flag_descriptions::kMacSyscallSandboxDescription, kOsMac,
      FEATURE_VALUE_TYPE(features::kMacSyscallSandbox)},
-    {"mac-v2-gpu-sandbox", flag_descriptions::kMacV2GPUSandboxName,
-     flag_descriptions::kMacV2GPUSandboxDescription, kOsMac,
-     FEATURE_VALUE_TYPE(features::kMacV2GPUSandbox)},
 #endif  // OS_MAC
 #if BUILDFLAG(IS_CHROMEOS_ASH) || defined(OS_WIN) || defined(OS_MAC)
     {"web-share", flag_descriptions::kWebShareName,
diff --git a/chrome/browser/flag_descriptions.cc b/chrome/browser/flag_descriptions.cc
index e80607f8a14af..37a4710d7163c 100644
--- a/chrome/browser/flag_descriptions.cc
+++ b/chrome/browser/flag_descriptions.cc
@@ -3715,10 +3715,6 @@ const char kMacSyscallSandboxName[] = "Mac Syscall Filtering Sandbox";
 const char kMacSyscallSandboxDescription[] =
     "Controls whether the macOS sandbox filters syscalls.";
 
-const char kMacV2GPUSandboxName[] = "Mac V2 GPU Sandbox";
-const char kMacV2GPUSandboxDescription[] =
-    "Controls whether the GPU process on macOS uses the V1 or V2 sandbox.";
-
 const char kMetalName[] = "Metal";
 const char kMetalDescription[] =
     "Use Metal instead of OpenGL for rasterization (if out-of-process "
diff --git a/chrome/browser/flag_descriptions.h b/chrome/browser/flag_descriptions.h
index 57bd6e08ddf48..337f0afb4add9 100644
--- a/chrome/browser/flag_descriptions.h
+++ b/chrome/browser/flag_descriptions.h
@@ -2156,9 +2156,6 @@ extern const char kImmersiveFullscreenDescription[];
 extern const char kMacSyscallSandboxName[];
 extern const char kMacSyscallSandboxDescription[];
 
-extern const char kMacV2GPUSandboxName[];
-extern const char kMacV2GPUSandboxDescription[];
-
 extern const char kMetalName[];
 extern const char kMetalDescription[];
 
diff --git a/content/browser/child_process_launcher_helper_mac.cc b/content/browser/child_process_launcher_helper_mac.cc
index 7dd338de3f738..fb0de8903f166 100644
--- a/content/browser/child_process_launcher_helper_mac.cc
+++ b/content/browser/child_process_launcher_helper_mac.cc
@@ -3,7 +3,6 @@
 // found in the LICENSE file.
 
 #include "base/command_line.h"
-#include "base/feature_list.h"
 #include "base/metrics/field_trial.h"
 #include "base/path_service.h"
 #include "base/posix/global_descriptors.h"
@@ -17,7 +16,6 @@
 #include "content/grit/content_resources.h"
 #include "content/public/browser/child_process_launcher_utils.h"
 #include "content/public/browser/content_browser_client.h"
-#include "content/public/common/content_features.h"
 #include "content/public/common/content_paths.h"
 #include "content/public/common/content_switches.h"
 #include "content/public/common/result_codes.h"
@@ -80,10 +78,7 @@ bool ChildProcessLauncherHelper::BeforeLaunchOnLauncherThread(
       command_line_->HasSwitch(sandbox::policy::switches::kNoSandbox) ||
       sandbox::policy::IsUnsandboxedSandboxType(sandbox_type);
 
-  bool use_v2 = (sandbox_type != sandbox::policy::SandboxType::kGpu) ||
-                base::FeatureList::IsEnabled(features::kMacV2GPUSandbox);
-
-  if (use_v2 && !no_sandbox) {
+  if (!no_sandbox) {
     // Generate the profile string.
     std::string profile =
         sandbox::policy::SandboxMac::GetSandboxProfile(sandbox_type);
diff --git a/content/gpu/gpu_main.cc b/content/gpu/gpu_main.cc
index cc0f150d08c13..b3afa0e60f45a 100644
--- a/content/gpu/gpu_main.cc
+++ b/content/gpu/gpu_main.cc
@@ -97,10 +97,8 @@
 #include "base/message_loop/message_pump_mac.h"
 #include "components/metal_util/device_removal.h"
 #include "components/metal_util/test_shader.h"
-#include "content/public/common/content_features.h"
 #include "media/gpu/mac/vt_video_decode_accelerator_mac.h"
 #include "sandbox/mac/seatbelt.h"
-#include "sandbox/policy/mac/sandbox_mac.h"
 #endif
 
 #if BUILDFLAG(USE_VAAPI)
@@ -155,7 +153,7 @@ class ContentSandboxHelper : public gpu::GpuSandboxHelper {
 #endif
 
 #if defined(OS_MAC)
-    if (base::FeatureList::IsEnabled(features::kMacV2GPUSandbox)) {
+    {
       TRACE_EVENT0("gpu", "Initialize VideoToolbox");
       media::InitializeVideoToolbox();
     }
diff --git a/content/public/common/content_features.cc b/content/public/common/content_features.cc
index b9ecf54987953..cb47933170ee9 100644
--- a/content/public/common/content_features.cc
+++ b/content/public/common/content_features.cc
@@ -970,9 +970,6 @@ const base::Feature kIOSurfaceCapturer{"IOSurfaceCapturer",
 const base::Feature kMacSyscallSandbox{"MacSyscallSandbox",
                                        base::FEATURE_DISABLED_BY_DEFAULT};
 
-const base::Feature kMacV2GPUSandbox{"MacV2GPUSandbox",
-                                     base::FEATURE_ENABLED_BY_DEFAULT};
-
 // Enables retrying to obtain list of available cameras on Macbooks after
 // restarting the video capture service if a previous attempt delivered zero
 // cameras.
diff --git a/content/public/common/content_features.h b/content/public/common/content_features.h
index 9e261f0e4e35b..2b397c2f0e703 100644
--- a/content/public/common/content_features.h
+++ b/content/public/common/content_features.h
@@ -226,7 +226,6 @@ CONTENT_EXPORT extern const base::Feature kWindowCaptureMacV2;
 CONTENT_EXPORT extern const base::Feature kDeviceMonitorMac;
 CONTENT_EXPORT extern const base::Feature kIOSurfaceCapturer;
 CONTENT_EXPORT extern const base::Feature kMacSyscallSandbox;
-CONTENT_EXPORT extern const base::Feature kMacV2GPUSandbox;
 CONTENT_EXPORT extern const base::Feature kRetryGetVideoCaptureDeviceInfos;
 #endif  // defined(OS_MAC)
 
diff --git a/testing/variations/fieldtrial_testing_config.json b/testing/variations/fieldtrial_testing_config.json
index 8062a4e3c2f8e..df56f51701810 100644
--- a/testing/variations/fieldtrial_testing_config.json
+++ b/testing/variations/fieldtrial_testing_config.json
@@ -3982,21 +3982,6 @@
             ]
         }
     ],
-    "MacV2GPUSandbox": [
-        {
-            "platforms": [
-                "mac"
-            ],
-            "experiments": [
-                {
-                    "name": "Enabled",
-                    "enable_features": [
-                        "MacV2GPUSandbox"
-                    ]
-                }
-            ]
-        }
-    ],
     "ManagedSigninPopup": [
         {
             "platforms": [